NickelMaghrebV1, Main, Exploration, bibRecord, 000772

Effects of nickel poisoning on expression pattern of the 72/73 and 94 kDa stress proteins in rat organs and in the COS‐7, HepG2, and A549 cell lines

Identifieur interne : 000772 ( Main/Exploration ); précédent : 000771; suivant : 000773

Effects of nickel poisoning on expression pattern of the 72/73 and 94 kDa stress proteins in rat organs and in the COS‐7, HepG2, and A549 cell lines

Auteurs : N. Hfaiedh [Tunisie] ; M. S. Allagui [Tunisie] ; A. El Feki [Tunisie] ; Y. Gaubin [France] ; J. C. Murat [France] ; J. P. Soleilhavoup [France] ; F. Croute [France]

Source :

Journal of Biochemical and Molecular Toxicology [ 1095-6670 ] ; 2005-03.

RBID : ISTEX:71FCEB75C3A1964C1B180CEDEAB9FECEE5796642

Descripteurs français

Wicri :
- topic : Nickel.

English descriptors

KwdEn :
- Animal Feed, Animals, Cell Line, Cell Proliferation (drug effects), Cercopithecus aethiops, Female, GRP94, Gene Expression Regulation (drug effects), HSC70 Heat-Shock Proteins, HSP70, HSP70 Heat-Shock Proteins (metabolism), HSP72 Heat-Shock Proteins, Heat-Shock Proteins (metabolism), Humans, Kidney (drug effects), Kidney (metabolism), Liver (drug effects), Liver (metabolism), Membrane Proteins (metabolism), Nickel, Nickel (poisoning), Ovary (drug effects), Ovary (metabolism), Rats, Rats, Wistar, food restriction.
MESH :
- chemical , metabolism : HSP70 Heat-Shock Proteins, Heat-Shock Proteins, Membrane Proteins.
- chemical , poisoning : Nickel.
- chemical : HSC70 Heat-Shock Proteins, HSP72 Heat-Shock Proteins.
- drug effects : Cell Proliferation, Gene Expression Regulation, Kidney, Liver, Ovary.
- metabolism : Kidney, Liver, Ovary.
- Animal Feed, Animals, Cell Line, Cercopithecus aethiops, Female, Humans, Rats, Rats, Wistar.

Abstract

The present study deals with the effects of Ni on the expression level of three stress proteins, namely, the cytosolic HSP72 and HSP73, and the reticulum‐associated GRP94. Experiments were carried out on “Wistar'' female rats daily injected with 4 mg NiCl2 per kg body weight for 1, 3, 5, and 10 days. Another set of experiments were carried out using cell lines, derived from the monkey kidney (COS‐7), and from human tumors of the lung (A549) and liver (HepG2). Cells were cultured for 4 days in the permanent presence of 100, 200, or 400 µM NiCl2. In control rats, stress proteins pattern was found to be tissue specific: two protein bands of 96 and 94 kDa were immunodetected with the anti‐GRP94 antibody in kidney and liver extracts, whereas only the 96 kDa band was present in ovary extracts. HSP73 was present in kidney, liver, and ovary whereas HSP72 was only found in kidney. In kidney of nickel‐treated animals, HSP73 and the 96 kDa proteins were overexpressed whereas HSP72 was strongly down regulated. No such effect was observed in liver or ovary. Similarly, in nickel‐treated cell lines, HSP72 was downregulated and GRP94 (96 kDa protein) was overexpressed. HSP73 expression appeared moderately increased in A549 cells but decreased in COS‐7 cells. Because long‐term caloric restriction was reported to reduce free radical generation in cells, the effect of 1 month food restriction (50%) was tested in rats as a possible way to lower oxidative damages induced by Ni. No significant effect on HSP expression was observed. © 2005 Wiley Periodicals, Inc. J Biochem Mol Toxicol 19:12–18, 2005; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.20056

Url:

https://api.istex.fr/document/71FCEB75C3A1964C1B180CEDEAB9FECEE5796642/fulltext/pdf

DOI: 10.1002/jbt.20056

Affiliations:

Le document en format XML

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<term>Cercopithecus aethiops</term>
<term>Female</term>
<term>GRP94</term>
<term>Gene Expression Regulation (drug effects)</term>
<term>HSC70 Heat-Shock Proteins</term>
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<term>Nickel (poisoning)</term>
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<front><div type="abstract" xml:lang="en">The present study deals with the effects of Ni on the expression level of three stress proteins, namely, the cytosolic HSP72 and HSP73, and the reticulum‐associated GRP94. Experiments were carried out on “Wistar'' female rats daily injected with 4 mg NiCl2 per kg body weight for 1, 3, 5, and 10 days. Another set of experiments were carried out using cell lines, derived from the monkey kidney (COS‐7), and from human tumors of the lung (A549) and liver (HepG2). Cells were cultured for 4 days in the permanent presence of 100, 200, or 400 µM NiCl2. In control rats, stress proteins pattern was found to be tissue specific: two protein bands of 96 and 94 kDa were immunodetected with the anti‐GRP94 antibody in kidney and liver extracts, whereas only the 96 kDa band was present in ovary extracts. HSP73 was present in kidney, liver, and ovary whereas HSP72 was only found in kidney. In kidney of nickel‐treated animals, HSP73 and the 96 kDa proteins were overexpressed whereas HSP72 was strongly down regulated. No such effect was observed in liver or ovary. Similarly, in nickel‐treated cell lines, HSP72 was downregulated and GRP94 (96 kDa protein) was overexpressed. HSP73 expression appeared moderately increased in A549 cells but decreased in COS‐7 cells. Because long‐term caloric restriction was reported to reduce free radical generation in cells, the effect of 1 month food restriction (50%) was tested in rats as a possible way to lower oxidative damages induced by Ni. No significant effect on HSP expression was observed. © 2005 Wiley Periodicals, Inc. J Biochem Mol Toxicol 19:12–18, 2005; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.20056</div>
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Serveur d'exploration sur le nickel au Maghreb

Effects of nickel poisoning on expression pattern of the 72/73 and 94 kDa stress proteins in rat organs and in the COS‐7, HepG2, and A549 cell lines

Effects of nickel poisoning on expression pattern of the 72/73 and 94 kDa stress proteins in rat organs and in the COS‐7, HepG2, and A549 cell lines

Source :

Descripteurs français

English descriptors

Abstract

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Le document en format XML

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Pour mettre un lien sur cette page dans le réseau Wicri

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